Oligonucleotides produced by digestion of KB cell ribosomal 5 S ribonucleic acid with specific nucleases.

Large Qligonucleotide Sequences in Wheat Germ Phenylalanine Transfer Ribonucleic Acid

The nucleotide sequence analysis of tRNA has required (a) identification of fragments isolated from complete digestion with pancreatic RNase and RNase T1 and, in addition, (b) the analysis of large oligonucleotide fragments produced by partial digestion with RNase T1 (l-4) and, in some cases, partial digestion with pancreatic RNase (5). In continuation of the work reported in the preceding pape...

Nucleotide sequence of U-2 ribonucleic acid. The sequence of the 5'-terminal oligonucleotide.

The nucleotide sequences were determined for the 5'-oligonucleotides obtained by complete pancreatic RNase digestion (P25) and complete T1 RNase digestion (T27) of U-2 RNA. Complete digestion of oligonucleotide P25 with snake venom phosphodiesterase produced pm3 2,2,7G, pAm, pUm, and pCp in approximately equimolar ratios. Partial digestion of these oligonucleotides with snake venom phosphodiest...

The nucleotide sequence of ribosomal 5 S ribonucleic acid from KB cells.

Maturation pathway for Novikoff ascites hepatoma 5.8 S ribosomal ribonucleic acid. Evidence for its presence in 32 S nuclear ribonucleic acid.

Evidence that 32 S nRNA contains 5.8 S rRNA was provided by studies on specific oligonucleotide sequences of these RNA species. Purified 32P-labeled 5.8 and 28 S rRNA and 32 S RNA were digested with T-1 ribonuclease, and the products were fractionated according to chain length by chromatography on DEAE-Sephadex A-25 at neutral pH. The oligonucleotides in Peak 8 were treated with alkaline phosph...

The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid.

It was first observed by Kozloff (1) that the deoxyribonuclease activity of cell-free extracts of Escherichiu coli could be increased by treatment with pancreatic ribonuclease. This stimulation was found to result from the destruction by the ribonuclease of an inhibitory ribonucleic acid. The phenomenon of cellular deoxyribonuclease inhibition is a widespread one, having been observed in yeast ...